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NadPrep NanoBlockers (for Illumina®, NXT)

Catalog Number:

#1006111 #1006112

NadPrep NanoBlockers (for Illumina®, NXT) is a universal blocking sequencing designed for use with Illumina's sequencing platform for the Nextera™ library. This product can bind to the Tn5 adapters sequences of the libraries, reducing non-specific binding between adapters, which enhances on-target rate, increasing the depth of enrichmen

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Features

Single-tube universal: Capable of blocking adapter libraries with single or dual 6-10 nt indexes.
Efficient blocking: Significantly enhances the efficiency of captured data.
Stable performance: Excellent and consistent capture performance across different batches and sizes of panels.
User-friendly: Compatible with Nanodigmbio's liquid-phase hybrid capture system

Workflow

Performance

Figure 1. The blocking efficiency of NadPrep NanoBlockers (for Illumina®, NXT). A total of 500 ng per library was used, with overnight hybridization of 16 hours, in combination with NadPrep Hybrid Capture Reagents and panels of various sizes. Under both single-plex (500 ng) and 12-plex (6000 ng) mixing schemes, the performance of NadPrep NanoBlockers (for Illumina®, NXT) remained excellent and stable across panels of different sizes. A. Mappability and on-target rate; B. Coverage uniformity and consistency; C. Fold 80

For research use only. Not for use in diagnostic procedures.

Catalog#	Color of Tube Cap	Component	Volume	Package/Storage
1006111		NadPrep NanoBlockers (for Illumina^®, NXT), 16 rxn	40 μL	-20℃
1006112		NadPrep NanoBlockers (for Illumina^®, NXT), 96 rxn	210 μL	-20℃

Can NadPrep NanoBlockers be diluted for use? What is the maximum total library input that can be blocked?

No. They can effectively block a total library input of up to 6 µg during hybridization capture when starting with 500 ng/library and performing 12 multiplexing cycles.

Product

Catalog#

NadPrep NanoBlockers (for Illumina^®, NXT), 16 rxn

1006111

NadPrep NanoBlockers (for Illumina^®, NXT), 96 rxn

1006112