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NAD Probes

NAD Probe is a one-stop service created by Nanodigmbio, covering the entire process from probe design, probe production, quality inspection, optimization, and result delivery. Through strict quality control (QC) and optimized NGS QC services, we can provide our customers with high-quality customized hybridization capture solutions, covering the application types in addition to conventional mutation detection, which include methylation capture, RNA capture, and virus capture.

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Features

Clarified sequence and known concentration for each probe

In compliance with the ISO13485 system production standard, providing uniform and stable results

Good uniformity of coverage, low GC bias

Support spike-in to other NadPanels or customized panels

Support hybridization capture of multiple samples

Content of Customized Service

Production And Quality Control

Independently Synthesized and Independently Inspected for Quality

Figure 1. Mass spectrometry results of a single probe from NAD Probes. The theoretical molecular weight was 38260.80 Da, and the mass spectrometry results showed that the measured molecular weight was 38266.1 Da.

Table 1. Examples for analysis detection of each probe.。

NGS QC

Table 2. Examples for NGS QC detection content and results.

Performance

Figure 2. Concentration deviation of different customized probe numbers.. One hundred tubes of customized probes were randomly selected, the number of probes varied from 10 to 17,000, and the probe concentration deviation was within ± 10%

Figure 3. Evaluation of the stability of customized probe synthesis by Probe Present. Different batches of customized probes were randomly selected, and the Probe Present results of multiple different batches of probes were above 99.9%.Note: Probe Present: The proportion of probe regions with average sequencing depth greater than 10x when the average sequencing depth is 200x.

Figure 4. Capture performance of DNACap panel.The library for human male genomic DNA (Promega, G1471) samples was constructed using the NadPrep® DNA Library Preparation Kit (for Illumina®). Three customized panels were respectively used to complete hybridization capture. Sequencing platform: Illumina NovaSeq 6000, PE 150. A. On-target rate, targeted region coverage, and Fold 80; B. Uniformity and consistency of coverage of targeted regions.

Figure 5. Capture performance of RNACap panel. The library for human male genomic DNA (Promega, G1471) samples was constructed using the NadPrep® DNA Library Preparation Kit (for Illumina®). The customized 500-gene RNACap panel was used to complete hybridization capture. Sequencing platform: Illumina NovaSeq 6000, PE 150. A. Coverage of regions with different GC content; B. Uniformity of coverage of targeted regions.

Figure 6. Capture performance of MethylCap panel. The library for Human male genomic DNA (Promega, G1471) samples after being transformed by the bisulfite method was constructed using the NadPrep® DNA Library Preparation Kit (for Illumina®). A customized 50 Kb MethylCap panel was used to complete hybridization capture. Sequencing platform: Illumina NovaSeq 6000, PE 150. A. Coverage of regions with different GC content; B. Uniformity of coverage of targeted regions.

For research use only. Not for use in diagnostic procedures.